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An analysis of chromosomal DNA using the Southern hybridization technique does not use:

  1. A
    Electrophoresis
  2. B
    Blotting
  3. C
    Autoradiography
  4. D
    PCR

Solution & Step-by-step Explanation

Southern blotting involves:Restriction digestion of DNA.Electrophoresis to separate fragments.Blotting (transfer) to a membrane.Hybridization with a radioactive probe.Autoradiography for detection.PCR is a technique for amplifying DNA and is not a part of the original Southern hybridization protocol.

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An analysis of chromosomal DNA using the Southern hybridization technique does not use:
A
Electrophoresis
B
Blotting
C
Autoradiography
D
PCR

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