During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
- ADenaturation
- BLigation
- CAnnealing
- DExtension
Solution & Step-by-step Explanation
The first step of PCR is Denaturation, which requires a high temperature (around 94
∘
C) to break the hydrogen bonds between the two DNA strands. If this temperature is not reached, the strands will not separate, and the subsequent steps (annealing and extension) cannot occur.
∘
C) to break the hydrogen bonds between the two DNA strands. If this temperature is not reached, the strands will not separate, and the subsequent steps (annealing and extension) cannot occur.