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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).Assertion (A): Polymerase chain reaction is used in DNA amplification.Reason (R): The ampicillin resistant gene is used as a selectable marker to check transformation.In the light of the above statements, choose the correct answer from the options given below:

  1. A
    Both (A) and (R) are correct and (R) is the correct explanation of (A)
  2. B
    Both (A) and (R) are correct but (R) is not the correct explanation of (A)
  3. C
    (A) is correct but (R) is not correct
  4. D
    (A) is not correct but (R) is correct

Solution & Step-by-step Explanation

1. Assertion (A) is correct: Polymerase Chain Reaction (PCR) is a technique used to make multiple copies (amplification) of a specific DNA segment.2. Reason (R) is correct: Antibiotic resistance genes, such as the ampicillin resistance gene (), are used as selectable markers in vector-mediated transformation to distinguish between transformants and non-transformants.However, the use of a selectable marker in transformation has nothing to do with the mechanism or purpose of DNA amplification via PCR. Therefore, (R) does not explain (A).

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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).Assertion (A): Polymerase chain reaction is used in DNA amplification.Reason (R): The ampicillin resistant gene is used as a selectable marker to check transformation.In the light of the above statements, choose the correct answer from the options given below:
A
Both (A) and (R) are correct and (R) is the correct explanation of (A)
B
Both (A) and (R) are correct but (R) is not the correct explanation of (A)
C
(A) is correct but (R) is not correct
D
(A) is not correct but (R) is correct

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