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hardMCQNEET 2024 R1@NUM: 1672026Zoology
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In the E. coli cloning vector pBR322, the role of 'ori' and 'rop' gene are:
image

  1. A
    'ori' is responsible for controlling the copy number of the linked DNA, and 'rop' for the protein involved in the replication of plasmid.
  2. B
    'ori' is for the protein involved in the replication of plasmid, and 'rop' for resistance to antibiotics.
  3. C
    'ori' is responsible for recognition sites, and 'rop' is responsible for antibiotic resistance.
  4. D
    'ori' is responsible for resistance to antibiotics, and 'rop' for the protein involved in the replication of plasmid.

Solution & Step-by-step Explanation

ori (Origin of replication): The sequence where replication starts; it also controls the copy number of the linked DNA.rop: Codes for the proteins involved in the replication of the plasmid.

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In the E. coli cloning vector pBR322, the role of 'ori' and 'rop' gene are:
image
A
'ori' is responsible for controlling the copy number of the linked DNA, and 'rop' for the protein involved in the replication of plasmid.
B
'ori' is for the protein involved in the replication of plasmid, and 'rop' for resistance to antibiotics.
C
'ori' is responsible for recognition sites, and 'rop' is responsible for antibiotic resistance.
D
'ori' is responsible for resistance to antibiotics, and 'rop' for the protein involved in the replication of plasmid.

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