Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.A. Insertion of recombinant DNA into the host cell.B. Cutting of DNA at specific location by restriction enzyme.C. Isolation of desired DNA fragment.D. Amplification of gene of interest using PCR.Choose the correct answer from the options given below:
- AB, C, D, A
- BC, A, B, D
- CC, B, D, A
- DB, D, A, C
Solution & Step-by-step Explanation
Shutterstock Explore The logical order for cloning a gene is:First, Cut the DNA with restriction enzymes (B).Isolate the specific fragment of interest (C).Amplify that fragment using PCR (D) to have enough material.Insert the resulting recombinant DNA into a host cell (A).(Wait, let me check the prompt's provided sequence: Option A is B, C, D, A. This is the standard sequence in NCERT).