Match the organism with its use in biotechnology.
| Column - I | Column - II |
| :--- | :--- |
| (a) Bacillus thuringiensis | (i) Cloning vector |
| (b) Thermus aquaticus | (ii) Construction of first rDNA molecule |
| (c) Agrobacterium tumefaciens | (iii) DNA polymerase |
| (d) Salmonella typhimurium | (iv) Cry proteins |
- A(a)-(ii), (b)-(iv), (c)-(iii), (d)-(i)
- B(a)-(iv), (b)-(iii), (c)-(i), (d)-(ii)
- C(a)-(iii), (b)-(ii), (c)-(iv), (d)-(i)
- D(a)-(iii), (b)-(iv), (c)-(i), (d)-(ii)
Solution & Step-by-step Explanation
Bacillus thuringiensis: Source of Cry proteins (Bt toxin).
Thermus aquaticus: Source of Taq DNA polymerase used in PCR.
Agrobacterium tumefaciens: Used as a cloning vector for plants (Ti plasmid).
Salmonella typhimurium: Used by Cohen and Boyer for the construction of the first rDNA molecule.
Thermus aquaticus: Source of Taq DNA polymerase used in PCR.
Agrobacterium tumefaciens: Used as a cloning vector for plants (Ti plasmid).
Salmonella typhimurium: Used by Cohen and Boyer for the construction of the first rDNA molecule.