Plasmid pBR322 has Pst I restriction enzyme site within gene amp^R that confers ampicillin resistance. If this enzyme is used for inserting a gene for - Biology MCQ NEET 2021 2026 | ExamTest.live

hardMCQNEET 20212026Biology

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Plasmid pBR322 has Pst I restriction enzyme site within gene am p^{R} that confers ampicillin resistance. If this enzyme is used for inserting a gene for β -galactosidase production and the recombinant plasmid is inserted in a E. coli strain:

A $it will lead to lysis of host cell$
B $it will be able to produce a novel protein with dual ability$
C $it will not be able to confer ampicillin resistance to the host cell$
D $the transformed cells will have the ability to resist ampicillin as well as produce β -galactosidase$

Solution & Step-by-step Explanation

If the foreign gene is inserted at the Pst I site, it will disrupt the am p^{R} gene (insertional inactivation). Consequently, the recombinant plasmid loses ampicillin resistance. The transformants will grow on tetracycline but not on ampicillin.

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Plasmid pBR322 has Pst I restriction enzyme site within gene am p^{R} that confers ampicillin resistance. If this enzyme is used for inserting a gene for β -galactosidase production and the recombinant plasmid is inserted in a E. coli strain:

it will lead to lysis of host cell

it will be able to produce a novel protein with dual ability

it will not be able to confer ampicillin resistance to the host cell

the transformed cells will have the ability to resist ampicillin as well as produce β -galactosidase

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