Which of the following is not a desirable feature of a cloning vector?
- APresence of origin of replication
- BPresence of a marker gene
- CPresence of single restriction enzyme site
- DPresence of two or more recognition sites
Solution & Step-by-step Explanation
'Ori' is needed for replication.Selectable markers are needed for screening.Single sites are preferred so the vector isn't fragmented into many pieces.Incorrect/Undesirable: Having multiple recognition sites for the same enzyme will cut the vector at many places, making cloning very complicated.