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easyMCQNEET 20222026Biology
1 mark

Which one of the following statements is not true regarding gel electrophoresis technique?

  1. A
    The process of extraction of separated DNA strands from gel is called elution
  2. B
    The separated DNA fragments are stained by using ethidium bromide
  3. C
    The presence of chromogenic substrate gives blue coloured DNA bands on the gel
  4. D
    Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light

Solution & Step-by-step Explanation

1. Elution is the recovery of DNA from the gel. True.2. Ethidium Bromide (EtBr) is the standard stain for DNA. True.3. Incorrect statement: Gel electrophoresis uses fluorescence (orange bands under UV after EtBr staining). Chromogenic substrates are used in blue-white screening for recombinants, not in standard electrophoresis.4. DNA-EtBr complex fluoresces orange under UV. True.

Practice this question

Try it yourself before checking the explanation above.

Which one of the following statements is not true regarding gel electrophoresis technique?
A
The process of extraction of separated DNA strands from gel is called elution
B
The separated DNA fragments are stained by using ethidium bromide
C
The presence of chromogenic substrate gives blue coloured DNA bands on the gel
D
Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light

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