The correct order of steps in Polymerase Chain Reaction () is:
- ADenaturation, Extension, Annealing
- BAnnealing, Extension, Denaturation
- CExtension, Denaturation, Annealing
- DDenaturation, Annealing, Extension
Solution & Step-by-step Explanation
Getty ImagesThe Polymerase Chain Reaction () proceeds in three distinct steps:Denaturation: The target is heated to about to separate the two strands.Annealing: The temperature is lowered to allow primers to bind to their complementary sequences on the single-stranded .Extension: polymerase adds nucleotides to the primers at about , synthesizing a new strand.