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The correct order of steps in Polymerase Chain Reaction () is:

  1. A
    Denaturation, Extension, Annealing
  2. B
    Annealing, Extension, Denaturation
  3. C
    Extension, Denaturation, Annealing
  4. D
    Denaturation, Annealing, Extension

Solution & Step-by-step Explanation

Getty ImagesThe Polymerase Chain Reaction () proceeds in three distinct steps:Denaturation: The target is heated to about to separate the two strands.Annealing: The temperature is lowered to allow primers to bind to their complementary sequences on the single-stranded .Extension: polymerase adds nucleotides to the primers at about , synthesizing a new strand.

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The correct order of steps in Polymerase Chain Reaction () is:
A
Denaturation, Extension, Annealing
B
Annealing, Extension, Denaturation
C
Extension, Denaturation, Annealing
D
Denaturation, Annealing, Extension

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