Biotechnology Questions

An important step in the manufacture of pulp for paper industry from the woody tissues of plants is the:

Production of a human protein in bacteria by genetic engineering is possible because:

There is a restriction endonuclease called EcoRI. What does the "co" part in it stand for?

Given below is a sample of a portion of DNA strand. What is so special shown in it?$5^{\prime }\text{ — GAATTC — }{3}^{\prime }$$3^{\prime }\text{ — CTTAAG — }{5}^{\prime }$

The process of RNA interference (RNAi) has been used in the development of plants resistant to:

Maximum number of existing transgenic animals is of:

Agarose extracted from sea weeds finds use in:

Which part would be most suitable for raising virus-free plants for micropropagation?

An analysis of chromosomal DNA using the Southern hybridization technique does not use:

The first human hormone produced by recombinant DNA technology is:

Which of the following is commonly used as a vector for cloning genes in higher plants?

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of:

DNA fragments generated by restriction endonucleases in a chemical reaction can be separated by:

Which of the following Bt crops is being grown in India by farmers?

Which one is a true statement regarding DNA polymerase used in PCR?

For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of:

In vitro clonal propagation in plants is characterized by:

PCR and Restriction Fragment Length Polymorphism (RFLP) are the methods for:

The figure below is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly identifies its certain component(s)?

A single strand of nucleic acid tagged with a radioactive molecule is called:

Consumption of which one of the following foods can prevent the kind of blindness associated with vitamin ‘A’ deficiency?

Which one of the following is a case of wrong matching?

What is it that forms the basis of DNA fingerprinting?

In genetic engineering, the antibiotics are used:

Biolistics (gene-gun) is suitable for:

Which of the following is not a feature of the plasmids?

Stirred-tank bioreactors have been designed for:

Which kind of therapy was given in 1990 to a four-year-old girl with adenosine deaminase (ADA) deficiency?

Which of the following restriction enzymes produces blunt ends?

Which of the following is not a component of downstream processing?

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using:

Which of the following is not required for any of the techniques of DNA fingerprinting available at present?

Which of the following is a restriction endonuclease?

The Taq polymerase enzyme is obtained from:

The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification?

The introduction of t-DNA into plants involves:

Golden rice is a genetically modified crop plant where the incorporated gene is meant for biosynthesis of:

The cutting of DNA at specific locations became possible with the discovery of:

The DNA molecules to which the gene of interest is integrated for cloning is called:

A protoplast is a cell:

To obtain virus-free healthy plants from a diseased one by tissue culture technique, which part/parts of the diseased plant will be taken?

Commonly used vectors for human genome sequencing are:

Which vector can clone only a small fragment of DNA?

Polyethylene glycol ($\text{PEG}$) method is used for:

Which one of the following is used as vector for cloning genes into higher organisms?

$\text{DNA}$ or $\text{RNA}$ segment tagged with a radioactive molecule is called:

Stirred-tank bioreactors have been designed for:

Transgenic plants are the ones:

Somaclones are obtained by:

The genetic defect — adenosine deaminase ($\text{ADA}$) deficiency may be cured permanently by:

What is true about $\text{Bt}$ toxin?

Which one of the following is commonly used in transfer of foreign $\text{DNA}$ into crop plants?

The genetically-modified ($\text{GM}$) brinjal in India has been developed for:

What is antisense technology?

Human insulin is being commercially produced from a transgenic species of:

The main objective of the production/use of herbicide-resistant $\text{GM}$ crops is to:

The linking of an antibiotic resistance gene with a plasmid vector became possible with:

Cry 1 endotoxins obtained from Bacillus thuringiensis are effective against:

Gel electrophoresis is used for:

A transgenic food crop which may help in solving the problem of night blindness in developing countries is:

A genetically engineered micro-organism used successfully in bioremediation of oil spills is a species of:

Silencing of $\text{mRNA}$ has been used in producing transgenic plants resistant to:

The first clinical gene therapy was given for treating:

Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produced (in the host cells):

Which one of the following is a correct statement?

A tumor inducing plasmid widely used in the production of transgenic plants is that of:

Electroporation procedure involves:

Assertion : In recombinant DNA technology, human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryote).Reason : Both bacteria and yeast multiply very fast to form huge populations which express the desired gene.

$Agrobacterium$ $tumefaciens$ is popular in genetic engineering because this bacterium is associated with the roots of all cereal and pulse crops.

$\text{Bacillus thuringiensis}$ forms protein crystals which contain insecticidal protein. This protein:

In cloning of cattle a fertilized egg is taken out of the mother's womb and:

Which one of the following techniques made it possible to genetically engineer living organisms?

Read the following four statements $(A-D)$. Which are the two statements having mistakes?$(A)$ The first transgenic buffalo Rosie produced milk which was human alpha-lactalbumin enriched.$(B)$ Restriction enzymes are used in isolation of $\text{DNA}$ from other macro-molecules.$(C)$ Downstream processing is one of the steps of $\text{R-DNA}$ technology.$(D)$ Disarmed pathogen vectors are also used in transfer of $\text{R-DNA}$ into the host.

Which one of the following palindromic base sequences in $\text{DNA}$ can be easily cut at about the middle by some particular restriction enzyme?

An improved variety of transgenic basmati rice:

Some of the characteristics of $\text{Bt}$ cotton are:

Restriction endonucleases are enzymes which:

Genetic engineering has been successfully used for producing: