biotechnology Questions

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:

Which of the following is not an application of PCR (Polymerase Chain Reaction)?

Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?

When gene targeting involving gene amplification is attempted in an individual's tissue to treat disease, it is known as:

DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as:

Match List-I with List-II:

DNA fingerprinting involves identifying differences in some specific regions in DNA sequence, called as:

It is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complementary DNA in a clone of cells, followed by its detection using autoradiography because:

Plasmid pBR322 has Pst I restriction enzyme site within gene $am{p}^R$ that confers ampicillin resistance. If this enzyme is used for inserting a gene for $\beta$ -galactosidase production and the recombinant plasmid is inserted in a E. coli strain:

With regard to insulin choose correct options:
(a) C-peptide is not present in mature insulin.
(b) The insulin produced by rDNA technology has C-peptide.
(c) The pro-insulin has C-peptide.
(d) A-peptide and B-peptide of insulin are interconnected by disulphide bridges.

Choose the correct answer from the options given below:

A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is:

During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?