biotechnology Questions

It is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complementary DNA in a clone of cells, followed by its detection using autoradiography because:

Match List-I with List-II:

Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?

When gene targeting involving gene amplification is attempted in an individual's tissue to treat disease, it is known as:

A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is:

Plasmid pBR322 has Pst I restriction enzyme site within gene $am{p}^R$ that confers ampicillin resistance. If this enzyme is used for inserting a gene for $\beta$ -galactosidase production and the recombinant plasmid is inserted in a E. coli strain:

With regard to insulin choose correct options:
(a) C-peptide is not present in mature insulin.
(b) The insulin produced by rDNA technology has C-peptide.
(c) The pro-insulin has C-peptide.
(d) A-peptide and B-peptide of insulin are interconnected by disulphide bridges.

Choose the correct answer from the options given below:

DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as:

Which of the following is not an application of PCR (Polymerase Chain Reaction)?

DNA fingerprinting involves identifying differences in some specific regions in DNA sequence, called as:

During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out: