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Biotechnology Questions

Practice 147 Biotechnology questions with detailed answers and explanations. Free MCQs, PYQs, and mock test questions for NEET, JEE, GATE, SSC and more.

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147
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92
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47
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8
Hard
Practice by Exam:Biology(131)BIOLOGY(8)Zoology(4)Botany(3)General Studies(1)

About Biotechnology — practice questions, PYQs & concepts

Biotechnology is a frequently-tested topic on Biology, BIOLOGY, Zoology. ExamTest.live currently lists 147 Biotechnology questions spanning previous-year papers from 2026 to 2026, each with worked solutions and a step-by-step explanation. Use the filters on this page to focus on a specific exam, difficulty level or year — every answer key is free, no sign-up required.

Biotechnology weight in competitive exams

Across our Biotechnology question bank, the largest sources are Biology (131), BIOLOGY (8), Zoology (4), Botany (3) and General Studies (1). Click any exam chip above to drill down to that exam-specific question set.

How to use this page

  • Start easy: 92 easy Biotechnology questions are available — build fluency before moving up.
  • Target weak years: Use the year filter to focus on the most recent 2026 paper or older PYQs you have not attempted.
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All Biotechnology Questions(1–100 of 147)

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Q1easymcqBiologyNEET (UG) 20172026
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The DNA fragments separated on an agarose gel can be visualised after staining with:
Q2easymcqBiologyNEET 20192026
0% accuracy
Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.
Q3easymcqBiologyNEET (UG) 20172026
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What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?
Q4easymcqBiologyNEET (UG) 20172026
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The process of separation and purification of expressed protein before marketing is called:
Q5easymcqBiologyNEET 20232026
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Expressed Sequence Tags (ESTs) refers to:
Q6easymcqBiologyNEET (UG) 20172026
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A gene whose expression helps to identify transformed cell is known as:
Q7mediummcqBiologyNEET 20192026
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What triggers activation of protoxin to active toxin of Bacillus thuringiensis in bollworm?
Q8easymcqBiologyNEET 20192026
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Which one of the following equipments is essentially required for growing microbes on a large scale, for industrial production of enzymes?
Q9easymcqBiologyNEET 20192026
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DNA precipitation out of a mixture of biomolecules can be achieved by treatment with:
Q10easymcqBiologyNEET 20192026
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Which of the following is true for Golden rice?
Q11mediummcqBiologyNEET 20232026
In tissue experiments, leaf mesophyll cells are put in a culture medium to form callus. This phenomenon may be called as:
Q12hardassertion/reasoningBotanyNEET 2024 R1@NUM: 1092026
Given
Q13easymcqBiologyNEET 20232026
In gene gun method used to introduce alien DNA into host cells, microparticles of _______ metal are used.
Q14hardmcqZoologyNEET 2024 R1@NUM: 1822026
Match List I with List II:
image
Q15easymcqBIOLOGYNEET 20192026
In Bt cotton, the Bt toxin present in plant tissue as protoxin is converted into active toxin due to:
Q16easymcqZoologyNEET 2024 R1@NUM: 1532026
The "Ti plasmid" of Agrobacterium tumefaciens stands for:
Q17easymcqZoologyNEET 2024 R1@NUM: 1652026
Which of the following statements is INCORRECT regarding bioreactors?
Q18easymcqBiologyNEET 20172026
The DNA fragments separated on an agarose gel can be visualised after staining with:
Q19easymcqBiologyNEET 20232026
Which of the following is not a cloning vector?
Q20easymcqBiologyNEET 20232026
During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:
Q21mediummcqBiologyNEET (UG) 20182026
The correct order of steps in Polymerase Chain Reaction () is:
Q22easymcqBiologyNEET 20222026
Given below are two statements:Statement I: Restriction endonucleases recognise specific sequences to cut DNA known as palindromic nucleotide sequences.Statement II: Restriction endonucleases cut the DNA strand a little away from the centre of the palindromic site.In the light of the above statements, choose the most appropriate answer from the options given below:
Q23easymcqGeneral StudiesAdda2472026
The CRISPR-Cas9 technology is used for:
Q24easymcqBiologyNEET 20232026
Upon exposure to UV radiation, DNA stained with ethidium bromide will show:
Q25hardmcqBiologyNEET 20222026
Statements related to human insulin are given below: Which statement(s) is/are correct about genetically engineered Insulin?(a) Pro-hormone insulin contain extra stretch of C-peptide.(b) A-peptide and B-peptide chains of insulin were produced separately in E. coli, extracted and combined by creating a disulphide bond between them.(c) Insulin used for treating Diabetes was extracted from Cattles and Pigs.(d) Pro-hormone insulin needs to be processed for converting into a mature and functional hormone.(e) Some patients develop allergic reactions to the foreign insulin.Choose the correct answer from the options given below:
Q26easymcqBIOLOGYNEET 20192026
In RNAi, the genes are silenced using:
Q27easymcqBiologyNEET 20172026
A gene whose expression helps to identify transformed cell is known as:
Q28mediummcqBiologyNEET 20222026
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).Assertion (A): Polymerase chain reaction is used in DNA amplification.Reason (R): The ampicillin resistant gene is used as a selectable marker to check transformation.In the light of the above statements, choose the correct answer from the options given below:
Q29easymcqBiologyNEET 20212026
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is:
Q30easymcqBiologyNEET (UG) 20182026
Which of the following is commonly used as a vector for introducing a fragment in human lymphocytes?
Q31easymcqBiologyNEET 20192026
Which part of the tobacco plant is infected by Meloidogyne incognita?
Q32mediummcqBiologyNEET 20222026
If a geneticist uses the blind approach for sequencing the whole genome of an organism, followed by assignment of functions to different segment, the methodology adopted by him is called as:
Q33hardmcqZoologyNEET 2024 R1@NUM: 1672026
In the E. coli cloning vector pBR322, the role of 'ori' and 'rop' gene are:
image
Q34easymcqBiologyNEET 20212026
DNA fingerprinting involves identifying differences in some specific regions in DNA sequence, called as:
Q35easymcqBiologyNEET 20172026
The process of separation and purification of expressed protein before marketing is called:
Q36easymcqBiologyNEET 20232026
Which one of the following techniques does not serve the purpose of early diagnosis of a disease for its early treatment?
Q37easymcqBiologyNEET 20232026
Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.A. Insertion of recombinant DNA into the host cell.B. Cutting of DNA at specific location by restriction enzyme.C. Isolation of desired DNA fragment.D. Amplification of gene of interest using PCR.Choose the correct answer from the options given below:
Q38mediummcqBotanyNEET 2024 R1@NUM: 1122026
Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:
Q39easymcqBiologyNEET (UG) 20182026
Use of bioresources by multinational companies and organisations without authorisation from the concerned country and its people is called:
Q40easymcqBiologyNEET 20222026
Which of the following is not a desirable feature of a cloning vector?
Q41mediummcqBiologyNEET 20212026
With regard to insulin choose correct options:
(a) C-peptide is not present in mature insulin.
(b) The insulin produced by rDNA technology has C-peptide.
(c) The pro-insulin has C-peptide.
(d) A-peptide and B-peptide of insulin are interconnected by disulphide bridges.

Choose the correct answer from the options given below:
Q42mediummcqBiologyNEET 20212026
Match List-I with List-II:
image
Q43easymcqBiologyNEET 20212026
When gene targeting involving gene amplification is attempted in an individual's tissue to treat disease, it is known as:
Q44easymcqBiologyNEET 20222026
DNA polymorphism forms the basis of:
Q45easymcqBiologyNEET 20222026
Which one of the following statements is not true regarding gel electrophoresis technique?
Q46easymcqBiologyNEET 20212026
During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
Q47mediummcqBiologyNEET 20222026
Transposons can be used during which one of the following?
Q48mediummcqBiologyNEET 20212026
It is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complementary DNA in a clone of cells, followed by its detection using autoradiography because:
Q49hardmcqBiologyNEET 20212026
Plasmid pBR322 has Pst I restriction enzyme site within gene that confers ampicillin resistance. If this enzyme is used for inserting a gene for -galactosidase production and the recombinant plasmid is inserted in a E. coli strain:
Q50easymcqBiologyNEET 20212026
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as:
Q51easymcqBiologyNEET 20222026
In the following palindromic base sequence of DNA, which one can be cut easily by a particular restriction enzyme?
Q52easymcqBiologyNEET 2020 E12026
The sequence that controls the copy number of the linked DNA in the vector, is termed:
Q53easymcqBiologyNEET 20212026
During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:
Q54mediummcqBiologyNEET 2020 E12026
Identify the wrong statement with regard to Restriction Enzymes.
Q55mediummcqBiologyNEET 2020 E12026
In gel electrophoresis, separated DNA fragments can be visualized with the help of:
Q56easymcqBiologyNEET 20212026
Which of the following is not an application of PCR (Polymerase Chain Reaction)?
Q57mediummcqBiologyNEET 2020 E12026
The specific palindromic sequence which is recognized by EcoRI is:
Q58mediummcqBiologyNEET 2020 E12026
Choose the correct pair from the following:
Q59mediummcqBotanyNEET 2024 R1@NUM: 1062026
What is the fate of a piece of DNA carrying only the gene of interest which is transferred into an alien organism?A. The piece of DNA would be able to multiply itself independently in the progeny cells of the organism.B. It may get integrated into the genome of the recipient.C. It may multiply and be inherited along with the host DNA.D. The alien piece of DNA is not an integral part of the chromosome.E. It shows the ability to replicate.
Q60easymcqBIOLOGYNEET 20192026
The two antibiotic resistance genes on vector are for:
Q61easymcqBIOLOGYNEET 20192026
A selectable marker is used to:
Q62mediummcqBIOLOGYNEET 20192026
Identify the incorrect statements pertaining to gel electrophoresis:(a) DNA is loaded on gel towards the anode.(b) Gel concentration does not affect DNA movement.(c) Smaller DNA fragments travel greater distances.(d) Pure DNA can be visualized directly by UV radiation.
Q63easymcqBiologyNEET (UG) 20182026
In India, the organisation responsible for assessing the safety of introducing genetically modified organisms for public use is:
Q64easymcqBiologyNEET 20172026
What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?
Q65easymcqBIOLOGYNEET 20192026
An enzyme catalysing the removal of nucleotides from ends of DNA is:
Q66mediummcqBiologyNEET 20222026
In gene therapy of Adenosine Deaminase (ADA) deficiency, the patient requires periodic infusion of genetically engineered lymphocytes because:
Q67easymcqBiologyNEET 20212026
Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?
Q68easymcqBIOLOGYNEET 20192026
The exploitation of bioresources of a nation by multinational companies without authorization from the concerned country is referred to as:
Q69easymcqBIOLOGYNEET 20192026
Match the following enzymes with their functions:Column I: (a) Restriction endonuclease, (b) Exonuclease, (c) DNA ligase, (d) Tag polymeraseColumn II: (i) joins DNA fragments, (ii) extends primers, (iii) cuts DNA at specific position, (iv) removes nucleotides from ends
Q70mediummcqBiologyBiology
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An important step in the manufacture of pulp for paper industry from the woody tissues of plants is the:
Q71easymcqBiologyBiology
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Production of a human protein in bacteria by genetic engineering is possible because:
Q72mediummcqBiologyBiology
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Agarose extracted from sea weeds finds use in:
Q73mediummcqBiologyBiology
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Maximum number of existing transgenic animals is of:
Q74mediummcqBiologyBiology
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The process of RNA interference (RNAi) has been used in the development of plants resistant to:
Q75mediummcqBiologyBiology
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Given below is a sample of a portion of DNA strand. What is so special shown in it?
Q76mediummcqBiologyBiology
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There is a restriction endonuclease called EcoRI. What does the "co" part in it stand for?
Q77easymcqBiologyBiology
To obtain virus-free healthy plants from a diseased one by tissue culture technique, which part/parts of the diseased plant will be taken?
Q78mediummcqBiologyBiology
Read the following four statements . Which are the two statements having mistakes? The first transgenic buffalo Rosie produced milk which was human alpha-lactalbumin enriched. Restriction enzymes are used in isolation of from other macro-molecules. Downstream processing is one of the steps of technology. Disarmed pathogen vectors are also used in transfer of into the host.
Q79mediummcqBiologyBiology
A protoplast is a cell:
Q80easymcqBiologyBiology
The DNA molecules to which the gene of interest is integrated for cloning is called:
Q81easymcqBiologyBiology
What is antisense technology?
Q82mediummcqBiologyBiology
Which one of the following techniques made it possible to genetically engineer living organisms?
Q83easymcqBiologyBiology
The cutting of DNA at specific locations became possible with the discovery of:
Q84easymcqBiologyBiology
Golden rice is a genetically modified crop plant where the incorporated gene is meant for biosynthesis of:
Q85mediummcqBiologyBiology
Silencing of has been used in producing transgenic plants resistant to:
Q86mediummcqBiologyBiology
The introduction of t-DNA into plants involves:
Q87easymcqBiologyBiology
Which of the following is not a feature of the plasmids?
Q88mediummcqBiologyBiology
In cloning of cattle a fertilized egg is taken out of the mother's womb and:
Q89mediummcqBiologyBiology
Polyethylene glycol () method is used for:
Q90mediummcqBiologyBiology
forms protein crystals which contain insecticidal protein. This protein:
Q91easymcqBiologyBiology
The Taq polymerase enzyme is obtained from:
Q92easymcqBiologyBiology
Which of the following is a restriction endonuclease?
Q93easymcqBiologyBiology
Which one of the following is commonly used in transfer of foreign into crop plants?
Q94mediummcqBiologyBiology
Which of the following is not required for any of the techniques of DNA fingerprinting available at present?
Q95easymcqBiologyBiology
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using:
Q96easymcqBiologyBiology
Which of the following is not a component of downstream processing?
Q97easymcqBiologyBiology
Which of the following restriction enzymes produces blunt ends?
Q98mediummcqBiologyBiology
A tumor inducing plasmid widely used in the production of transgenic plants is that of:
Q99mediummcqBiologyBiology
A genetically engineered micro-organism used successfully in bioremediation of oil spills is a species of:
Q100mediummcqBiologyBiology
What is true about toxin?
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Biotechnology questions are commonly asked in Biology, BIOLOGY, Zoology, Botany, General Studies. You can filter by exam using the links above.
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Start with easy questions to build confidence, then tackle medium and hard ones. Use the filter buttons to sort by difficulty. Reveal the answer only after attempting each question, and read the explanation thoroughly.